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71.
利用频率为2.0 MHz,强度为5.0 W/cm2的超声激活血卟啉对在体小鼠艾氏腹水瘤细胞 进行了抑癌作用研究.通过对一次和多次处理的肿瘤体积和重量变化趋势的研究,发现声动力疗法 对艾氏腹水瘤细胞增殖有显著抑制作用,且多次处理比一次处理效果更为明显. 相似文献
72.
73.
WUKefu MAXiaotong 《科学通报(英文版)》2003,48(22):2519-2522
Proinflammation represents a pathophysiological state on the early stage of a number of diseases, especially the infectious and immunological ones. In recent years, proinflammation has attracted much attention, and the term 損roinflammation factors?appears frequently in the literature. While investigating leukemia and leukemic cells from the angle of 損roinflammation state? we got some intriguing findings, e.g. we detected the significantly elevated expression of proinflammation factor IL-18 in patients with acute myeloid leukemia (AML), which could up-regulate matrix metalloproteinases (MMP) and specific tissue inhibitors (TIMPs). The increased MMP may play a role in the aggressiveness of myeloid leukemic cells, and be associated with a poor prognosis. This phenomenon reflects an ignored aspect of leukemia. Investigations from the angle of 損roinflammation state?have broaden the fields of tumor and leukemia study. 相似文献
74.
FANGJianwu HUANGSiluo LIUHuisheng M.Crepin XUTao LIUJianfeng 《科学通报(英文版)》2003,48(15):1539-1547
Fibroblast growth factor-2 (FGF-2) is a member of a large family of proteins that bind heparin and heparan sulfate and modulate the function of a wide range of cell types. It has been proved that FGF-2 stimulates the growth and development of new blood vessels (angiogenesis) that contribute to the pathogenesis of several diseases (i.e. cancer, atherosclerosis). However, many of the biological activities of FGF-2 have been found to depend on its receptor抯 intrinsic tyrosine kinase activity and second messengers such as the mitogen activated protein kinases. This review will focus on the mechanism of FGF-2/FGFR induced signaling pathway in tumor and human breast cancer. 相似文献
75.
The Ras family of GTPases in cancer cell invasion 总被引:3,自引:0,他引:3
Hernández-Alcoceba R del Peso L Lacal JC 《Cellular and molecular life sciences : CMLS》2000,57(1):65-76
The ability of tumoral cells to invade surrounding tissues is a prerequisite for metastasis. This is the most life-threatening
event of tumor progression, and so research is intensely focused on elucidating the mechanisms responsible for invasion and
metastasis. The Ras superfamily of GTPases comprises several subfamilies of small GTP-binding proteins whose functions include
the control of proliferation, differentiation, and apoptosis, as well as cytoskeleton organization. The development of metastasis
is a multistep process that requires coordinated activation of proliferation, motility, changes in normal cell-to-cell and
cell-to-substrate contacts, degradation of extracellular matrix, inhibition of apoptosis, and adaptation to an inappropriate
tissue environment. Several members of the Ras superfamily of proteins have been implicated in these processes. The present
review summarizes the current knowledge in this field. 相似文献
76.
HSP70在肺癌组织中表达水平的研究 总被引:3,自引:0,他引:3
采用免疫组织化学SABC法检测39例肺部组织中HSP70的表达。结果显示:HSP70在高度分化的肺鳞癌和肺腺癌细胞中的阳性表达率与癌旁正常肺组织之间具有高度显著性差异(P〈0.01),HSP70的表达在高分化的肺鳞癌与肺腺癌之间则无显著性差异(P〉0.05),在低分化的肺鳞癌和肺腺癌组织中,HSP70呈阴性反应,HSP70在肺癌组织中表达水平的高低与肺癌细胞的分化程度密切相关,而与肺癌的组织学类型 相似文献
77.
Jun Yang Ying Wu Ping Ma Yanhua Zhao Ying You Susan E. Kane Ning Mao Yangpei Zhang 《科学通报(英文版)》2000,45(15):1390-1394
A series of retro viral vectors encoding humanmdr1 gene alone as wetl as in combination with either humanmgmt gene or human mutantSer
31-dhfr gene are engineered. The resultant retroviruses are used to transduce human umbilical cord blood CD34+ cetls. It has been shown that expression of dual drug resistance genes in transduced cetls confers a broad range of resistance
to both kinds of corresponding drugs. These data suggest a rationale for the use of such double chemoresistance gene constructs
in anin vivo model in which transduced hematopoietic cetls will acquire multiple protection against the cytotoxic side effects of combination
chemotherapy and may have future application in chemoprotection of normal tissues, thus killing tumor cetls more effectivety. 相似文献
78.
用ICP-AES法测定了44例恶性肿瘤患者和33例健康人血清Cu、Zn、Ca、Mg、Se含量.结果显示:恶性肿瘤组血清Ca、Se含量低于健康组,血清Cu含量高于健康组,两组相比较,均有非常显著差异(P<0.01).提示上述元素可能与恶性肿瘤的发生、发展有一定关系. 相似文献
79.
CCN蛋白家族研究进展 总被引:1,自引:1,他引:1
CCN蛋白家族包含多个成员,它们结构相似,富含半胱氮酸。作为分泌蛋白,CCN蛋白能够促进细胞生长、细胞粘附、细胞迁移;诱导细胞凋亡;并调控肿瘤生长、血管发生和软骨内骨化。由于CCN蛋白在癌症和其它疾病预测或/和诊断中的重要性,成为近几年研究的热点。本文将简述近几年在CCN蛋白领域的研究进展。 相似文献
80.
HAb18G/CD147-mediated calcium mobilization and hepatoma metastasis require both C-terminal and N-terminal domains 总被引:4,自引:0,他引:4
Jiang JL Chan HC Zhou Q Yu MK Yao XY Lam SY Zhu H Ho LS Leung KM Chen ZN 《Cellular and molecular life sciences : CMLS》2004,61(16):2083-2091
HAb18G/CD147 is a heavily glycosylated protein containing two immunoglobulin superfamily domains. Our previous studies have indicated that overexpression of HAb18G/CD147 enhances metastatic potentials in human hepatoma cells by disrupting the regulation of store-operated Ca2+ entry by nitric oxide (NO)/cGMP. In the present study, we investigated the structure-function of HAb18G/CD147 by transfecting truncated HAb18G/CD147 fragments into human 7721 hepatoma cells. The inhibitory effect of HAb18G/CD147 on 8-bromo-cGMP-regulated thapsigargin-induced Ca2+ entry was reversed by the expression of either C or N terminus truncated HAb18G/CD147 in T7721C and T7721N cells, respectively. The potential effect of HAb18G/CD147 on metastatic potentials, both adhesion and invasion capacities, of hepatoma cells was abolished in T7721C cells, but not affected in T7721N cells. Release and activation of matrix metalloproteinases (MMPs), MMP-2 and MMP-9, were found to be enhanced by the expression of HAb18G/CD147, and this effect was abolished by both truncations. Thapsigargin significantly enhanced release and activation of MMPs (MMP-2 and MMP-9) in non-transfected 7721 cells, and this effect was negatively regulated by SNAP. However, no effects of thapsigargin or SNAP were observed in T7721 cells, and expression of HAb18G/CD147 enhanced secretion and activation of MMPs at a stable and high level. Taken together, these results suggest that both ectodomain and intracellular domains of HAb18G/CD147 are required to mediate the effect of HAb18G/CD147 on the secretion and activation of MMPs and metastasis-related processes in human hepatoma cells by disrupting the regulation of NO/cGMP-sensitive intracellular Ca2+ mobilization although each domain may play different roles.Received 1 April 2004; received after revision 15 June 2004; accepted 22 June 2004 相似文献